mouse lif 250-02 Search Results


mouse lif 250-02  (PeproTech)
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PeproTech mouse lif 250-02
Screening of <t>anti-LIF</t> scFvs by phage display. ( A ) Identification of the binding selectivity of 14 clones by phage ELISA. Phage clones binding to human LIF (black bars) and <t>mouse</t> <t>LIF</t> (gray bars) were detected by the HRP-conjugated anti-M13 phage antibody. ( B ) Binding kinetics of 7 phage supernatants with human LIF (red) and mouse LIF (black) were measured by Gator. BSA was used as a negative control
Mouse Lif 250 02, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Screening of anti-LIF scFvs by phage display. ( A ) Identification of the binding selectivity of 14 clones by phage ELISA. Phage clones binding to human LIF (black bars) and mouse LIF (gray bars) were detected by the HRP-conjugated anti-M13 phage antibody. ( B ) Binding kinetics of 7 phage supernatants with human LIF (red) and mouse LIF (black) were measured by Gator. BSA was used as a negative control

Journal: BMC Immunology

Article Title: Antagonist anti-LIF antibody derived from naive human scFv phage library inhibited tumor growth in mice

doi: 10.1186/s12865-024-00636-w

Figure Lengend Snippet: Screening of anti-LIF scFvs by phage display. ( A ) Identification of the binding selectivity of 14 clones by phage ELISA. Phage clones binding to human LIF (black bars) and mouse LIF (gray bars) were detected by the HRP-conjugated anti-M13 phage antibody. ( B ) Binding kinetics of 7 phage supernatants with human LIF (red) and mouse LIF (black) were measured by Gator. BSA was used as a negative control

Article Snippet: The other recombinant protein sources were as follows: human LIF (Z02681, Genscript, China), biotinylated human LIF (LIF-H82E2, Acrobiosystems, China), mouse LIF (250-02, PeproTech, USA), cynomolgus LIF (RP1074Y-100, Kingfisher Biotech, USA), human LIFR-his (10,628-H08H, Sino Biological, China), and human gp130-his (10,974-HCCH, Sino Biological, China).

Techniques: Binding Assay, Clone Assay, Enzyme-linked Immunosorbent Assay, Negative Control

Expression and screening of recombinant monoclonal antibodies. ( A ) SDS-PAGE analysis of the purified antibodies under reducing conditions. M, molecular weight marker. ( B ) Dose dependent binding of antibodies to immobilized human LIF and mouse LIF, normal mouse IgG was used as a negative control. ( C ) Blocking activities of antibodies (2 µg/mL) were analyzed by competitive ELISA, n = 3

Journal: BMC Immunology

Article Title: Antagonist anti-LIF antibody derived from naive human scFv phage library inhibited tumor growth in mice

doi: 10.1186/s12865-024-00636-w

Figure Lengend Snippet: Expression and screening of recombinant monoclonal antibodies. ( A ) SDS-PAGE analysis of the purified antibodies under reducing conditions. M, molecular weight marker. ( B ) Dose dependent binding of antibodies to immobilized human LIF and mouse LIF, normal mouse IgG was used as a negative control. ( C ) Blocking activities of antibodies (2 µg/mL) were analyzed by competitive ELISA, n = 3

Article Snippet: The other recombinant protein sources were as follows: human LIF (Z02681, Genscript, China), biotinylated human LIF (LIF-H82E2, Acrobiosystems, China), mouse LIF (250-02, PeproTech, USA), cynomolgus LIF (RP1074Y-100, Kingfisher Biotech, USA), human LIFR-his (10,628-H08H, Sino Biological, China), and human gp130-his (10,974-HCCH, Sino Biological, China).

Techniques: Expressing, Recombinant, Bioprocessing, SDS Page, Purification, Molecular Weight, Marker, Binding Assay, Negative Control, Blocking Assay, Competitive ELISA

Characterization of antibody 1G11. ( A ) SPR affinity analysis of 1G11 binding to human, cynomolgus and mouse LIF. ( B ) Competitive ELISA and SPR analysis of 1G11. 1G11 competes with LIFR for binding to LIF, while 1G11 does not compete with gp130 for binding to LIF. ( C ) Western-bolt analysis for the inhibitory effect of 1G11 on LIF-induced p-STAT3

Journal: BMC Immunology

Article Title: Antagonist anti-LIF antibody derived from naive human scFv phage library inhibited tumor growth in mice

doi: 10.1186/s12865-024-00636-w

Figure Lengend Snippet: Characterization of antibody 1G11. ( A ) SPR affinity analysis of 1G11 binding to human, cynomolgus and mouse LIF. ( B ) Competitive ELISA and SPR analysis of 1G11. 1G11 competes with LIFR for binding to LIF, while 1G11 does not compete with gp130 for binding to LIF. ( C ) Western-bolt analysis for the inhibitory effect of 1G11 on LIF-induced p-STAT3

Article Snippet: The other recombinant protein sources were as follows: human LIF (Z02681, Genscript, China), biotinylated human LIF (LIF-H82E2, Acrobiosystems, China), mouse LIF (250-02, PeproTech, USA), cynomolgus LIF (RP1074Y-100, Kingfisher Biotech, USA), human LIFR-his (10,628-H08H, Sino Biological, China), and human gp130-his (10,974-HCCH, Sino Biological, China).

Techniques: Binding Assay, Competitive ELISA, Western Blot